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For the first step, we reasoned that basal locomotion in a sensory-deprived environment would most closely reflect the unperturbed, ongoing activity of Drosophila AS circuits.
Therefore, we used these behaviors as a target dataset for neural network generation. In our initial experiments, we observed that some flies could remain stationary for over 20 min. Therefore, to capture the complete range of behavioral intervals, we acquired 5 h of basal locomotor sequences from Canton-S strain flies ( Fig 3A).